Silica runs a virtual insilico PCR with a set of primers on a selected genome. Multiple primer pairs are allowed, off target products will be shown if present. All primer combinations are tested, provided sufficiend binding to the DNA and reasonable amplicon size.
Primers can be provided in fasta format or uploaded as a fasta file (*.fa).
The "Load Example" button loads an example primer set. Hit "Launch Analysis" to try the functionality.