Load table file
Select column separator for import
Table after import
Load predefined settings
Settings to reshape the table
Settings to extract the fluorescence data block
Settings to extract data
Table after reshaping
Create RDML file
|PCR Format - Columns:|
|PCR Format - Column Label:|
|PCR Format - Rows:|
|PCR Format - Row Label:|
|PCR Format - Predefined:|
Complement columns 1 to 5 (required)
Table for export
The columns 1-6 must be filled for successfull import into RDML. Leave them free for empty wells.
White rows indicate missing information, only green rows are complete for import.
RDML-TableShaper is a tool to shape tables with amplification data for the import with RDML-tools. This is error-prone and second best to original RDML export by the machine. As some machines do not offer RDML export, TableShaper might help. Most qPCR machines export Excel files. Please convert them by opening in Excel (Microsoft) or Calc (Libre Office), selecting the table with the amplification data and save as tab separated text file. The tab separated text file can be modified using TableShaper.
The use of TableShaper
TableShaper follows three consecutive steps:
1. The table separators have to be identified on the first tab. If it does not work out automatically, the separator has to be selected manually. The table columns need to be recognized in the table below.
2. The content need to be recognized in the second tab. If your table format is not found in the predefined settings, you may iteratively select the parameters and load / save them for later use. Adapt the "Number of columns to keep" and "Number of rows to keep" to match the experiments cycle and well numbers and avoid cutoff. "Settings to reshape the table" is the most dramatic shaping option. You can opt to flip the table or to build a table from value list. The result is drawn below and can be used to select the columns for data extraction.
3. Fill the missing content in the third tab. To be able to import the table into RDML, the first six columns have to be filled. Using the fields on the third tab eases this step as squares B2-D7 can be used. Take care in this step as errors in the target id and sample id can not be fixed in RDML later. Save the result as .tsv file.
4. To create an RDML file fill the information on the "Create RDML file" card and hit the button. RDML-Edit will open with the created RDML file and allow to save or view the RDML file.
Alternatively the data can be imported int existing RDML files. Open the RDML file in RDML-Edit (or create new) and enable editing with the "enable edit mode" button. Now change to the experiments tab and open an experiment or create a new experiment. In the experiment, create a new run. Take care to set the ID and PCR-Format fields correctly. Now select the exported .tsv file in "Import Amplification Data". Once saved you can inspect the result using RunView or save it as RDML file on the first tab.
The table files can be provided in text format with tab, comma or semicolon as separator(*.txt, *.tsv, and *.csv).
The "Show Example" button loads an example table file (click to download file) from a BioRad iCycler v9.35.
A general help file is available as RDML-Tools Help.