Padlock: Design padlock probes for in-situ sequencing

Ensembl gene name(s)

Probe arm length

Edit/Hamming distance

Use hamming distance (default is edit distance):

Apply distance to entire probe (default is probe arm):

Allow overlapping probes (default is non-overlapping):

Select genome

Padlock is a method to design probes for in-situ sequencing.

You need to input Ensembl gene IDs (e.g., ENSG00000232810). Gene symbols are currently not supported.

The "Show Example" button loads an example reference genome with matching Ensembl gene identifiers.