Tracy: basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files

Tracy is an efficient and versatile command-line application to basecall, align, assemble and deconvolute Sanger Chromatogram trace files. Tracy supports various Chromatogram input file formats (*.ab1, *.abi, *.scf), aligns traces across entire genomes, calls variants and supports as output commonly used NGS file formatics (VCF/BCF, JSON). Tracy's companion web applications enable an interactive exploration of results. All code is open-source and hosted on Tracy's GitHub page.

Contents:

Installation
Usage
Web Application
FAQ

Tips

For questions, help or feature requests please contact gear_genomics@embl.de

If you use tracy in your work please cite:

Rausch, T., Fritz, M.H., Untergasser, A. and Benes, V.
Tracy: basecalling, alignment, assembly and deconvolution of sanger chromatogram trace files.
BMC Genomics 21, 230 (2020).
https://doi.org/10.1186/s12864-020-6635-8